Methadone determination in puparia and its effect on the development of Lucilia sericata (Diptera, Calliphoridae).
نویسندگان
چکیده
This paper describes a sensitive UPLC-MS/MS method for quantification of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) in single empty puparial case of Lucilia sericata. Larvae were reared on substrates spiked with different concentrations of methadone (0-4 μg/g). Methadone was quantified in puparia reared on high concentrated substrates (0.8-4 μg/g). The major metabolite of methadone (EDDP) was not detected, confirming rapid elimination of metabolites by the larvae before pupation. The effects of methadone on the development of L. sericata were also investigated. No effect on sex ratio was detected. A significant difference was calculated for emerged adults but no trends could be observed. Concerning the developmental curve, a significant difference was observed between control and high methadone concentrations using the Kolmogorov-Smirnov test.
منابع مشابه
چکیده گزارش میاز محوطه دهانی ناشی از Lucilia sericata (Diptera: Calliphoridae) در یک راس بز در استان کرمان
Lucilia sericata یکی از انگلهای خارجی اختیاری است که میتواند باعث میاز در دامها و ندرتا در انسان بشوند. عفونت در انسان و دامهای علفخوار در محل زخمها اتفاق میافتد که میتواند منجر به خارش و التهاب محل ضایعه بشود. گزارش حاضر در رابطه با یک راس بز چهار ساله با علائم بیاشتهایی، لاغری پیشرونده، کاهش هوشیاری و کما بوده که تعداد بسیار زیادی لارو زنده مگس L. sericata از زخم های موجود در محوطه د...
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ورودعنوان ژورنال:
- Forensic science international
دوره 209 1-3 شماره
صفحات -
تاریخ انتشار 2011